Plant Signaling

As multicellular organisms, plants constantly perceive their environment and respond appropriately, which is required for their survival, as well as the coordination of their growth and development. In particular, we are focusing on cell surface-localised immune receptors, called pattern recognition receptors, that perceive microbe-derived or endogenous patterns to activate innate immune responses.

Plant pattern recognition receptors are receptor kinases or are part of receptor kinase complexes, and thus offer exquisite model systems to study the molecular basis of plant immunity, but also the mechanisms underlying the function, activation and regulation receptor kinase-mediated pathways in growth, development and responses to environmental stresses. In addition, we are interested in identifying and engineering immune receptors to improve disease resistance in crops. 

• The Arabidopsis pattern recognition receptor EFR enhances fire blight resistance in apple
  Piazza, Stefano; Campa, Manuela; Pompili, Valerio; Dalla Costa, Lorenza; Salvagnin, Umberta; et al.
  Horticulture Research 10.1038/s41438-021-00639-3  DEC 2021
• Molecular mechanisms of early plant pattern-triggered immune signaling (vol 81, pg 3449, 2021)
  DeFalco, Thomas A.; Zipfel, Cyril
  Molecular Cell 10.1016/j.molcel.2021.09.028 OCT 21 2021
• Family-wide evaluation of RAPID ALKALINIZATION FACTOR peptides
  Abarca, Alicia; Franck, Christina M.; Zipfel, Cyril
  Plant Physiology 10.1093/plphys/kiab308  OCT 2021
• Review Molecular mechanisms of early plant pattern-triggered immune signaling
  DeFalco, Thomas A.; Zipfel, Cyril
  Molecular Cell 10.1016/j.molcel.2021.07.029  SEP 2 2021
• Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activate Arabidopsis innate immunity two independent mechanisms
  Schellenberger, Romain; Crouzet, Jerome; Nickzad, Arvin; Shu, Lin-Jie; Kutschera, Alexander; et al.
  Proceedings Of The National Academy Of Sciences Of The United States Of America 10.1073/pnas.2101366118  SEP 28 2021
• Activation loop phosphorylaton of a non-RD receptor kinase initiates plant innate immune signaling
  Bender, Kyle W.; Couto, Daniel; Kadota, Yasuhiro; Macho, Alberto P.; Sklenar, Jan; et al.
  Proceedings Of The National Academy Of Sciences Of The United States Of America10.1073/pnas.2108242118  SEP 21 2021
• Importance of tyrosine phosphorylation for transmembrane signaling in plants
  Muehlenbeck, Henning; Bender, Kyle W.; Zipfel, Cyril
  Biochemical Journal 10.1042/BCJ20210202  JUL 2021
• Perception of a divergent family of phytocytokines by the Arabidopsis receptor kinase MIK2
  Rhodes, Jack; Yang, Huanjie; Moussu, Steven; Boutrot, Freddy; Santiago, Julia; Zipfel, Cyril
  Nature Communications 10.1038/s41467-021-20932-y  JAN 29 2021
• An evergreen mind and a heart for the colors of fall
  Sylvain Aubry, Bastien Christ, Bernhard Kräutler, Enrico Martinoia, Howard Thomas, Cyril Zipfel
  Journal of Experimental Botany, https://doi.org/10.1093/jxb/erab162
• The Arabidopsis immune receptor EFR increases resistance to the bacterial pathogens Xanthomonas and Xylella in transgenic sweet orange
  Mitre, Leticia Kuster; Teixeira-Silva, Natalia Sousa; Rybak, Katarzyna; Magalhaes, Diogo Maciel; de Souza-Neto, Reinaldo Rodrigues; et al.
  Plant Biotechnology Journal 10.1111/pbi.13629  JUL 2021
• Large-scale identification of ubiquitination sites on membrane-associated proteins in Arabidopsis thaliana seedlings
  Grubb, Lauren E.; Derbyshire, Paul; Dunning, Katherine E.; Zipfel, Cyril; Menke, Frank L. H.; et al.
  Plant Physiology 10.1093/plphys/kiab023  APR 2021
• A novel allele of the Arabidopsis thaliana MACPF protein CAD1 results in deregulated immune signaling
  Holmes, Danalyn R.; Bredow, Melissa; Thor, Kathrin; Pascetta, Sydney A.; Sementchoukova, Irina; et al.
  Genetics 10.1093/genetics/iyab022  APR 2021
• Wheat Pm4 resistance to powdery mildew is controlled by alternative splice variants encoding chimeric proteins
  Sanchez-Martin, Javier; Widrig, Victoria; Herren, Gerhard; Wicker, Thomas; Zbinden, Helen; Gronnier, Julien; Sporri, Laurin; Praz, Coraline R; Heuberger, Matthias; Kolodziej, Markus C.; Isaksson, Jonatan; Steuernagel, Burkhard; Karafiatova, Miroslava; Dolezel, Jaroslav; Zipfel, Cyril; Keller, Beat
  Nature Plants,  10.1038/s41477-021-00869-2 MAR 2021
• The transcriptional landscape of Arabidopsis thaliana pattern-triggered immunity
  Bjornson, Marta; Pimprikar, Priya; Nuernberger, Thorsten; Zipfel, Cyril
  Nature Plants   DOI: 10.1038/s41477-021-00874-5  MAR 2021
• A membrane-bound ankyrin repeat protein confers race-specific leaf rust disease resistance in wheat
  Kolodziej, Markus C.; Singla, Jyoti; Sanchez-Martin, Javier; Zbinden, Helen; Simkova, Hana; Karafiatova, Miroslava; Dolezel, Jaroslav; Gronnier, Julien; Poretti, Manuel; Glauser, Gaetan; Zhu, Wangsheng; Koster, Philipp; Zipfel, Cyril; Wicker, Thomas; Krattinger, Simon; Keller, Beat
  Nature Communications,  DOI: 10.1038/s41467-020-20777-x FEB 11 2021

• A receptor-like protein mediates plant immune responses to herbivore-associated molecular patterns
  Steinbrenner, Adam D.; Munoz-Amatriain, Maria; Chaparro, Antonio F.; Aguilar-Venegas, Jessica Montserrat; Lo, Sassoum; Okuda, Satohiro; Glauser, Gaetan; Dongiovanni, Julien; Shi, Da; Hall, Marlo; Crubaugh, Daniel; Holton, Nicholas; Zipfel, Cyril; Abagyan, Ruben; Turlings, Ted C. J.; Close, Timothy J.; Huffaker, Alisa; Schmelz, Eric A
  Proceedings Of The National Academy Of Sciences Of The United States Of America 117(49): 31510-31518   DOI: 10.1073/pnas.2018415117   DEC 8 2020
• Lumi-Map, a Real-Time Luciferase Bioluminescence Screen of Mutants Combined with MutMap, Reveals Arabidopsis Genes Involved in PAMP-Triggered Immunity
  Kato, Hiroaki; Onai, Kiyoshi; Abe, Akira; Shimizu, Motoki; Takagi, Hiroki; et al.
  Molecular Plant-microbe Interactions   DOI: 10.1094/MPMI-05-20-0118-TA  DEC 2020
• The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity
  Thor, Kathrin; Jiang, Shushu; Michard, Erwan; George, Jeoffrey; Scherzer, Soenke; et al.
  Nature  DOI: 10.1038/s41586-020-2702-1, SEP 24 2020
• Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants
  Cheval, Cecilia; Samwald, Sebastian; Johnston, Matthew G.; de Keijzer, Jeroen; Breakspear, Andrew; et al.
  Proceedings Of The National Academy Of Sciences Of The United States Of America,  DOI: 10.1073/pnas.1907799117 APR 28 2020
• Update on Receptors and Signaling
  Cheung, Alice Y.; Qu, Li-Jia; Russinova, Eugenia; Zhao, Yunde; Zipfel, Cyril
  Plant Physiology DOI: 10.1104/pp.20.00275, APR 2020
• Carbonic anhydrases CA1 and CA4 function in atmospheric CO2-modulated disease resistance
  Zhou, Yeling; Vroegop-Vos, Irene A.; Van Dijken, Anja J. H.; Van der Does, Dieuwertje; Zipfel, Cyril; et al.
  Planta  DOI: 10.1007/s00425-020-03370-w  MAR 7 2020
• CrRLK1L receptor-like kinases HERK1 and ANJEA are female determinants of pollen tube reception
  Galindo-Trigo, Sergio; Blanco-Tourinan, Noel; DeFalco, Tomas A.; Wells, Eloise S.; Gray, Julie E.; et al.
  Embo Reports, 10.15252/embr.201948466, FEB 2020
• …

• Plant innate immunity
• Plant receptor kinases
• Phosphorylation-based regulation of plant signaling
• Plasma membrane organization
• Cell wall integrity sensing  
• Plant biotechnology 

• Protein-protein interactions
• Protein phosphorylation analysis
• Plant-bacteria interactions
• Bioimaging
• Signaling bioassays in immunity, growth and development
• Electrophysiology