Evolutionary Genetics

Most plants face attacks by fungal pathogens. In agriculture, outbreaks of diseases are frequent and pose a significant threat to sustainable food production. What enables pathogens to overcome host defenses and cause damage is poorly understood. A key evolutionary step for pathogens is to evolve effectors that specifically target and disable the plant immune system.

Our group uses experimental and population genomics tools to analyze the rapid emergence of pathogens. A major focus is to use genome-wide association studies (GWAS) to directly identify the genes in pathogen genomes linked to the breakdown of host resistance. We also assemble complete genomes to analyze mechanisms that generate high degrees of sequence polymorphism. We are particularly interested in the action of transposable elements (selfish elements in the genome) and the evolution of gene regulation. Our group also conducts population genomic studies to analyze the process of adaptive evolution in natural field populations.

• The rise and fall of genes: origins and functions of plant pathogen pangenomes
  Badet, Thomas; Croll, Daniel
  Current Opinion In Plant Biology  DOI: 10.1016/j.pbi.2020.04.009  AUG 2020
• A Chromosome-Scale Genome Assembly for the Fusarium oxysporum Strain Fo5176 To Establish a Model Arabidopsis-Fungal Pathosystem
  Fokkens, Like; Guo, Li; Dora, Susanne; Wang, Bo; Ye, Kai; Sanchez-Rodriguez, Clara; Croll, Daniel
  G3-genes Genomes Genetics   DOI: 10.1534/g3.120.401375  OCT 2020
• Transcriptome plasticity underlying plant root colonization and insect invasion by Pseudomonas protegens
  Vesga, Pilar; Flury, Pascale; Vacheron, Jordan; Keel, Christoph; Croll, Daniel; Maurhofer M
  Isme Journal  DOI: 10.1038/s41396-020-0729-9    NOV 2020
• Comparative Genomics Analyses of Lifestyle Transitions at the Origin of an Invasive Fungal Pathogen in the Genus Cryphonectria
  Stauber, Lea; Prospero, Simone; Croll, Daniel
  Msphere  DOI: 10.1128/mSphere.00737-20  SEP-OCT 2020
• Maintenance of variation in virulence and reproduction in populations of an agricultural plant pathogen
  Dutta, Anik; Croll, Daniel; McDonald, Bruce A.; Barrett, Luke G.
  Evolutionary Applications  DOI: 10.1111/eva.13117 SEP 2020
• Genome compartmentalization predates species divergence in the plant pathogen genus Zymoseptoria
  Feurtey, Alice; Lorrain, Cecile; Croll, Daniel; Eschenbrenner, Christoph; Freitag, Michael; et al.
  Bmc Genomics  DOI: 10.1186/s12864-020-06871-w  AUG 26 2020
• Natural selection drives population divergence for local adaptation in a wheat pathogen
  Pereira, Danilo; Croll, Daniel; Brunner, Patrick C.; McDonald, Bruce A.
  Fungal Genetics And Biology  DOI: 10.1016/j.fgb.2020.103398  AUG 2020
• A Chromosome-Scale Genome Assembly for the Fusarium oxysporum Strain Fo5176 To Establish a Model Arabidopsis-Fungal Pathosystem
  Fokkens, Like; Guo, Li; Dora, Susanne; Wang, Bo; Ye, Kai; Sanchez-Rodriguez, Clara; Croll, Daniel
  G3-genes Genomes Genetics 10.1534/g3.120.401375 OCT 2020
• A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization
  Sanchez-Vallet, Andrea; Tian, Hui; Rodriguez-Moreno, Luis; Valkenburg, Dirk-Jan; Saleem-Batcha, Raspudin; et al.
  Plos Pathogens  DOI: 10.1371/journal.ppat.1008652  JUN 2020
• Chromosomal assembly and analyses of genome-wide recombination rates in the forest pathogenic fungus Armillaria ostoyae
  Heinzelmann, Renate; Rigling, Daniel; Sipos, Gyorgy; Muensterkoetter, Martin; Croll, Daniel
  Heredity  DOI: 10.1038/s41437-020-0306-z   MAR 2020
• Purging of highly deleterious mutations through severe bottlenecks in Alpine ibex
  Grossen, Christine; Guillaume, Frederic; Keller, Lukas F.; Croll, Daniel
  Nature Communications,  11(1) Number: 1001  DOI: 10.1038/s41467-020-14803-1  FEB 21 2020
• Stress-Driven Transposable Element De-repression Dynamics and Virulence Evolution in a Fungal Pathogen
  Fouche, Simone; Badet, Thomas; Oggenfuss, Ursula; Plissonneau, Clemence; Francisco, Carolina Sardinha; Croll Daniel
  Molecular Biology And Evolution  DOI: 10.1093/molbev/msz216  JAN 2020
• A 19-isolate reference-quality global pangenome for the fungal wheat pathogen Zymoseptoria tritici
  Badet, Thomas; Oggenfuss, Ursula; Abraham, Leen; McDonald, Bruce A.; Croll, Daniel
  Bmc Biology 18(1) Article Number: 12 DOI: 10.1186/s12915-020-0744-3  FEB 11 2020
• Complex Evolutionary Origins of Specialized Metabolite Gene Cluster Diversity among the Plant Pathogenic Fungi of the Fusarium graminearum Species Complex
  Tralamazza, Sabina Moser; Rocha, Liliana Oliveira; Oggenfuss, Ursula; Correa, Benedito; Croll, Daniel
  Genome Biology And Evolution, 10.1093/gbe/evz225, NOV 2019
• The emergence of the multi-species NIP1 effector in Rhynchosporium was accompanied by high rates of gene duplications and losses
  Mohd-Assaad, N; McDonald, BA; Croll, D
  ENVIRONMENTAL MICROBIOLOGY, 21 (8):2677-2695; 10.1111/1462-2920.14583 AUG 2019
• Cautionary Notes on Use of the MoT3 Diagnostic Assay for Magnaporthe oryzae Wheat and Rice Blast Isolates
  Gupta, DR; Avila, CSR; Win, J; Soanes, DM; Ryder, LS; Croll, D; Bhattacharjee, P; Hossain, MS; Mahmud, NU; Mehebub, MS; Surovy, MZ; Rahman, MM; Talbot, NJ; Kamoun, S; Islam, MT
  PHYTOPATHOLOGY, 109 (4):504-508; 10.1094/PHYTO-06-18-0199-LE APR 2019
• Wheat blast: from its origins in South America to its emergence as a global threat
  Ceresini, PC; Castroagudin, VL; Rodrigues, FA; Rios, JA; Aucique-Perez, CE; Moreira, SI; Croll, D; Alves, E; de Carvalho, G; Maciel, JLN; McDonald, BA
  MOLECULAR PLANT PATHOLOGY, 20 (2):155-172; 10.1111/mpp.12747 FEB 2019
• Rapid Parallel Evolution of Azole Fungicide Resistance in Australian Populations of the Wheat Pathogen Zymoseptoria tritici
  McDonald, MC; Renkin, M; Spackman, M; Orchard, B; Croll, D; Solomon, CPS; Milgate, A
  APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 85 (4):10.1128/AEM.01908-18 FEB 2019
• The birth and death of effectors in rapidly evolving filamentous pathogen genomes
  Fouche, S; Plissonneau, C; Croll, D
  CURRENT OPINION IN MICROBIOLOGY, 46 34-42; 10.1016/j.mib.2018.01.020 DEC 2018
• The Monothiol Glutaredoxin Grx4 Regulates Iron Homeostasis and Virulence in Cryptococcus neoformans
  Attarian, R; Hu, GG; Sanchez-Leon, E; Caza, M; Croll, D; Do, E; Bach, H; Missall, T; Lodge, J; Jung, WH; Kronstad, JW
  MBIO, 9 (6):10.1128/mBio.02377-18 NOV-DEC 2018
• Wheat Blast: Past, Present, and Future
  Ceresini, PC; Castroagudin, VL; Rodrigues, FA; Rios, JA; Aucique-Perez, CE; Moreira, SI; Alves, E; Croll, D; Maciel, JLN
  Edited by: Leach JE; Lindow SE
  ANNUAL REVIEW OF PHYTOPATHOLOGY, VOL 56, 56 427-456; 10.1146/annurev-phyto-080417-050036  2018
• The Genome Biology of Effector Gene Evolution in Filamentous Plant Pathogens
  Sanchez-Vallet, A; Fouche, S; Fudal, I; Hartmann, FE; Soyer, JL; Tellier, A; Croll, D Edited by: Leach JE; Lindow SE
  ANNUAL REVIEW OF PHYTOPATHOLOGY, VOL 56, 56 21-40; 10.1146/annurev-phyto-080516-035303   2018
• Genomewide signatures of selection in Epichloe reveal candidate genes for host specialization
  Schirrmann, Melanie K; Zoller, Stefan; Croll, Daniel; Stukenbrock, EH; Leuchtmann, A; Fior, S
  MOLECULAR ECOLOGY   Volume: 27   Issue: 15   Pages: 3070-3086 10.1111/mec.14585   AUG 2018

• Genetic basis of pathogen virulence
• Ecological genomics and local adaptation of pathogens
• Population genomics of pathogen outbreaks
• Structure and evolution of pathogen genomes
• Transposable elements and the evolution of gene regulation

• Crop resistance breeding
• Bioinformatics and genome assembly
• Molecular plant pathology
• Fungal community analyses
• Genome-wide association mapping (GWAS)