Structural Biology & Cell Biology & Genetics

Plants are nature’s other successful experiment with multicellular life. We are interested in signaling and metabolic pathways that allow plants to grow and to develop. Our approach combines structural biology and biochemistry with cell biology and genetics in Arabidopsis.

Currently, we study how plant cells communicate with each other at their cell membrane, where they have evolved unique membrane receptor kinases. These receptors sense steroid and peptide hormones to orchestrate growth and development, to establish and maintain stem cell populations and to form plant organs and tissues. We have shown that these receptors require helper proteins for activation. We now want to obtain a molecular understanding of plant peptide hormone signaling and identify novel receptor complexes.

A second line of research is to understand how plants sense and store phosphate, an essential plant nutrient. We became interested in this topic by identifying a protein complex, which uses cytosolic ATP to generate long chains of inorganic polyphosphate, an important phosphate store in yeast. We have initiated a set of projects to test whether plants also make use of polyphosphates to store phosphate.

• MacroH2A histone variants limit chromatin plasticity through two distinct mechanisms
  Kozlowski, M; Corujo, D; Hothorn, M; Guberovic, I; Mandemaker, IK; Blessing, C; Sporn, J; Gutierrez-Triana, A; Smith, R; Portmann, T; Treier, M; Scheffzek, K; Huet, S; Timinszky, G; Buschbeck, M; Ladurner, AG
  EMBO REPORTS, 19 (10):10.15252/embr.201744445 OCT 2018
• CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis
  Anne, Pauline; Amiguet-Vercher, Amelia; Brandt, Benjamin; Kalmbach, Lothar; Geldner, Niko; Hothorn, Michael; Hardtke, Christian S
  DEVELOPMENT   Volume: 145   Issue: 10     Article Number: UNSP dev162354  https://doi.org/10.1242/dev.162354 MAY 2018
• The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling
  Ulrich Hohmann, Joël Nicolet, Andrea Moretti, Ludwig A. Hothorn & Michael Hothorn
  Nature Plants (2018),  https://doi.org/10.1038/s41477-018-0150-9
• Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors
  Hohmann, U; Santiago, J; Nicolet, J; Olsson, V; Spiga, FM; Hothorn, LA; Butenko, MA; Hothorn, M
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 115 (13):3488-3493; https://doi.org/10.1073/pnas.1714972115 MAR 27 2018
• Control of plant phosphate homeostasis by inositol pyrophosphates and the SPX domain 
  Jung, Ji-Yul; Ried, Martina K.; Hothorn, Michael; et al. 
  CURRENT OPINION IN BIOTECHNOLOGY, 49: 156-162; https://doi.org/10.1016/j.copbio.2017.08.012 FEB 2018
• Perception of root-active CLE peptides requires CORYNE function in the phloem vasculature 
  Hazak, Ora; Brandt, Benjamin; Cattaneo, Pietro; Santiago, J; Rodriguez-Villalon, A; Hothorn, M; Hardtke, CS
  EMBO REPORTS, 18 (8): 1367-1381 https://doi.org/10.15252/embr.201643535 AUG 2017
• The Structural Basis of Ligand Perception and Signal Activation by Receptor Kinases 
  Hohmann, Ulrich; Lau, Kelvin; Hothorn, Michael 
  ANNUAL REVIEW OF PLANT BIOLOGY, VOL 68, 68: 109-137 2017 Review; Book Chapter (Details)
• The macro domain as fusion tag for carrier-driven crystallization 
  Wild, Rebekka; Hothorn, Michael 
  PROTEIN SCIENCE, 26 (2): 365-374 FEB 2017 Document Type: Article (Details)
• Control of eukaryotic phosphate homeostasis by inositol polyphosphate sensor domains 
  Wild, Rebekka; Gerasimaite, Ruta; Jung, Ji-Yul; et al. 
  SCIENCE, 352 (6288): 986-990 MAY 20 2016 Document Type: Article (Details)

• Plant membrane signaling
• Receptor kinases
• Brassinosteroid signaling
• Cytokinin signaling
• Polyphosphate metabolism
• Phosphate sensing

• Receptor agonist/antagonist development
• Structural Biology / Protein Crystallography of signaling proteins and metabolic enzymes
• Quantitative biochemistry. Protein-Protein interactions. Protein-Ligand interactions