Our laboratory focuses on various aspects of a highly conserved mechanism called “RNA silencing”, which encompasses many processes in which tiny RNA molecules (20-30 nt long) are used as guides to promote nucleotide sequence-specific regulations at the levels of mRNA stability and translation, and, in some organisms, at the level of DNA methylation and chromatin modification.

More specifically, we are interested in understanding the biogenesis, modes of action and biological functions of two main classes of cellular small RNAs that are both processed from double-stranded RNA precursors by RNase III-like enzymes in the Dicer family, and loaded into Argonaute (AGO) proteins that effect their activity at the RNA and DNA levels, as part of RNA-induced silencing complexes (RISCS).

► Google Scholar: https://scholar.google.ch/citations?hl=de&user=a1D7YVUAAAAJ

• A contemporary reassessment of the enhanced transient expression system based on the tombusviral silencing suppressor protein P19
  F Jay, F Brioudes, O Voinnet
  The Plant Journal 113 (1), 186-204, 2023 10.1111/tpj.16032
• Revisiting small RNA movement in plants
  O Voinnet
  Nature Reviews Molecular Cell Biology 23 (3), 163-164, 202210.1038/s41580-022-00455-0
• HASTY, the Arabidopsis EXPORTIN5 ortholog, regulates cell‐to‐cell and vascular microRNA movement
  F Brioudes, F Jay, A Sarazin, T Grentzinger, EA Devers, O Voinnet
  The EMBO Journal 40 (15), doi: 10.15252/embj.2020107455 (2021)
• A universal method for the rapid isolation of all known classes of functional silencing small RNAs
  Grentzinger, Thomas; Oberlin, Stefan; Schott, Gregory; Handler, Dominik; Svozil, Julia; et al.
  Nucleic Acids Research  DOI: 10.1093/nar/gkaa472  AUG 20 2020
• Movement and differential consumption of short interfering RNA duplexes underlie mobile RNA interference
  Devers, EA; Brosnan, CA; Sarazin, A; Albertini, D; Amsler, AC; Brioudes, F; Jullien, PE; Lim, P; Schott, G; Voinnet, O
  Nature Plants 2020 10.1038/s41477-020-0687-2
• Functional characterization of Arabidopsis ARGONAUTE 3 in reproductive tissues
  Jullien, Pauline E.; Grob, Stefan; Marchais, Antonin; Pumplin, Nathan; Chevalier, Clement; et al.
  Plant Journal 2020 10.1111/tpj.14868
• Chemical enhancers of posttranscriptional gene silencing in Arabidopsis
  Jay, F; Vitel, M; Brioudes, F; Louis, M; Knobloch, T; Voinnet, O
  RNA, 25 (9):1078-1090; 10.1261/rna.068627.118 SEP 2019
• Extensive profiling in Arabidopsis reveals abundant polysome-associated 24-nt small RNAs including AGO5-dependent pseudogene-derived siRNAs
  Marchais, A; Chevalier, C; Voinnet, O
  RNA, 25 (9):1098-1117; 10.1261/rna.069294.118 SEP 2019
• Genome-scale, single-cell-type resolution of microRNA activities within a whole plant organ
  Brosnan, CA; Sarazin, A; Lim, P; Bologna, NG; Hirsch-Hoffmann, M; Voinnet, O
  EMBO JOURNAL, 38 (13):10.15252/embj.2018100754 JUL 1 2019
• RNA Sequencing identifies an IGA vasculitis associated serum microRNA signature, discriminating patients with IGA vasculitisfrom age- and sex-matched healthy subjects
  Hocevar, A; Lakota, K; Grentzinger, T; Sarazin, A; Brezovec, N; Kuret, T; Distler, O; Cucnik, S; Voinnet, O; Sodin-Semrl, S; Tomsic, M; Frank-Bertoncelj, M
  ANNALS OF THE RHEUMATIC DISEASES, 78 1192-1193; 2 10.1136/annrheumdis-2019-eular.6874 JUN 2019