Molecular Genetics and Evolution

Our work focuses on the mycorrhizal symbiosis between plants and arbuscular mycorrhizal (AM) fungi. The symbiosis occurs in the majority of terrestrial plant species. Through the effects of the fungus on plant phosphorus acquisition, plants benefit and can grow better when forming the symbiosis. The association is ancient and our group seeks to understand the genetics and evolution of these important fungi and how AM fungal genetics can influence plant growth.

We have demonstrated three features of AM genetics that have important consequences for how plants grow. First, the fungi harbour genetically different nuclei in a common cytoplasm. Second, the fungi can fuse and the nuclei can mix giving rise to genetically novel AM fungi. Third, segregation of nuclei occurs during spore formation, giving rise to genetically different AM fungi. Both mixing of nuclei and segregation have important conseqences on the growth of rice. We manipulate the genetics of AM fungi using these processes to study which genes are affected in the plant by genetic changes in the fungus. These processes are natural and involve no gene insertion. A part of our work involves applying this technology to produce new strains of AM fungi for commerical inoculation of crops in Colombia (in collaboration with the National University of Colombia).

• Dual RNA-seq reveals large-scale non-conserved genotype x genotype-specific genetic reprograming and molecular crosstalk in the mycorrhizal symbiosis
  Mateus, ID; Masclaux, FG; Aletti, C; Rojas, EC; Savary, R; Dupuis, C; Sanders, IR
  ISME JOURNAL, 13 (5):1226-1238; 10.1038/s41396-018-0342-3 MAY 2019
• Variation in allele frequencies at the bg112 locus reveals unequal inheritance of nuclei in a dikaryotic isolate of the fungus Rhizophagus irregularis
  Masclaux, Frederic G.; Wyss, Tania; Mateus-Gonzalez, Ivan D.; Aletti, Consolee; Sanders, Ian R.
  MYCORRHIZA, 28 (4):369-377; 10.1007/s00572-018-0834-z MAY 2018
• Sex, plasticity, and biologically significant variation in one Glomeromycotina species
  Sanders I.R.
  New Phytologist, 10.1111/nph.15049   2018
• Within-species phylogenetic relatedness of a common mycorrhizal fungus affects evenness in plant communities through effects on dominant species
  Romain Savary, Lucas Villard, Ian R. Sanders
  PLOS ONE, https://doi.org/10.1371/journal.pone.0198537  2018
• A population genomics approach shows widespread geographical distribution of cryptic genomic forms of the symbiotic fungus Rhizophagus irregularis 
  Savary, Romain; Masclaux, Frederic G.; Wyss, Tania; et al. 
  ISME JOURNAL, 12 (1): 17-30 10.1038/ismej.2017.153  JAN 2018
• Cost-efficient production of in vitro Rhizophagus irregularis 
  Rosikiewicz, Pawel; Bonvin, Jeremy; Sanders, Ian R. 
  MYCORRHIZA, 27 (5): 477-486 10.1007/s00572-017-0763-2  JUL 2017
• Bacteria with Phosphate Solubilizing Capacity Alter Mycorrhizal Fungal Growth Both Inside and Outside the Root and in the Presence of Native Microbial Communities.
  Ordoñez Y.M., Fernandez B.R., Lara L.S., Rodriguez A., Uribe-Vélez D., Sanders I.R.,
  2016. PloS one, 11 (6) pp. e0154438.  10.1371/journal.pone.0154438
• Colombian-Swiss Research to Help Feed the Planet: From Green Revolution to Microbial Revolution
  Rodriguez Alia, Sanders Ian,
  Acta Biologica Colombiana, 21 pp. 297-303.  http://dx.doi.org/10.15446/abc.v21n1sup.50856 2016.
  
• Aligning molecular studies of mycorrhizal fungal diversity with ecologically important levels of diversity in ecosystems 
  Sanders, Ian R.; Rodriguez, Alia 
  ISME JOURNAL, 10 (12): 2780-2786 DEC 2016 Article (Details)
• Population genomics reveals that within-fungus polymorphism is common and maintained in populations of the mycorrhizal fungus Rhizophagus irregularis 
  Wyss, Tania; Masclaux, Frederic G.; Rosikiewicz, Pawel; et al. 
  ISME JOURNAL, 10 (10): 2514-2526 OCT 2016 Article (Details)
• Bacteria with Phosphate Solubilizing Capacity Alter Mycorrhizal Fungal Growth Both Inside and Outside the Root and in the Presence of Native Microbial Communities 
  Marcela Ordonez, Yuli; Rocio Fernandez, Belen; Susana Lara, Lidia; et al. 
  PLOS ONE, 11 (6): JUN 2 2016 Article (Details)

• Genetics and evolutionary genetics of AM fungi 
• Influence of AM fungal genetics on the symbiosis with plants
• Importance of AM fungal diversity on plant communities
• Biotechnological application of AM fungi in tropical agriculture in Colombia

• Molecular genetics
• Ecology
• Evolution
• Agronomy
• Plant – microbe interactions