Microbiology

Plants do not exist as sterile organisms but are populated by microorganisms both below (rhizosphere) and above the ground (phyllosphere). We are interested in a systems-level understanding of the phyllosphere with the aim to identify organizing principles of bacterial community members. We developed a cultivation-independent metaproteogenomic approach and applied it to phyllosphere communities of different host plants in planta in order to get an unbiased view on the overall bacterial community composition and in particular to identify abundant proteins as a proxy for important protein function of community members in the habitat.

Such analyses are paralleled by work using synthetic microbial communities colonizing Arabidopsis thaliana. We are testing indigenous commensal bacteria and their interaction with the foliar plant pathogen Pseudomonas syringae pv. tomato DC3000 on the model plant and investigate underlying mechanisms of plant protection.

• Mapping phyllosphere microbiota interactions in planta to establish genotype-phenotype relationships
  Schaefer, Martin; Vogel, Christine M.; Bortfeld-Miller, Miriam; Mittelviefhaus, Maximilian; Vorholt, Julia A.
  Nature Microbiology  10.1038/s41564-022-01132-w  JUN 2022
• Mass spectrometry-based approaches to study lanthanides and lanthanide-dependent proteins in the phyllosphere
  Hemmerle, Lucas; Ochsner, Andrea M.; Vonderach, Thomas; Hattendorf, Bodo; Vorholt, Julia A.
  Rare-earth Element Biochemistry: Methanol Dehydrogenases And Lanthanide Biology 10.1016/bs.mie.2021.01.006  2021
• Protective role of the Arabidopsis leaf microbiota against a bacterial pathogen
  Vogel, Christine M.; Potthoff, Daniel B.; Schafer, Martin; Barandun, Niculo; Vorholt, Julia A.
  Nature Microbiology 10.1038/s41564-021-00997-7  DEC 2021
• Capacity of soil bacteria to reach the phyllosphere and convergence of floral communities despite soil microbiota variation
  Massoni, Julien; Bortfeld-Miller, Miriam; Widmer, Alex; Vorholt, Julia A.
  Proceedings of the National Academy of Sciences of the United States of America  10.1073/pnas.2100150118  OCT 12 2021
• Structural diversity of the coenzyme methylofuran and identification of enzymes for the biosynthesis of its polyglutamate side chain
  Hemmann, Jethro L.; Bruehwiler, Manuel R.; Bortfeld-Miller, Miriam; Vorholt, Julia A.
  Journal Of Biological Chemistry 10.1016/j.jbc.2021.100682  JAN-JUN 2021
• A general non-self response as part of plant immunity
  Maier BA, Kiefer P, Field CM, Hemmerle L, Bortfeld-Miller M, Emmenegger B, Schäfer M, Pfeilmeier S, Sunagawa S, Vogel CM, and Vorholt JA.
  Nature Plants 7: 696–705 (2021). doi: 10.1038/s41477-021-00913-1.
• The plant NADPH oxidase RBOHD is required for microbiota homeostasis in leaves.
  Pfeilmeier S, Petti GC, Bortfeld-Miller M, Daniel B, Field CM, Sunagawa S, and Vorholt JA.
  Nat Microbiol (2021). doi: 10.1038/s41564-021-00929-5.
• Bradyrhizobium diazoefficiens Requires Chemical Chaperones To Cope with Osmotic Stress during Soybean Infection
  Ledermann, Raphael; Emmenegger, Barbara; Couzigou, Jean-Malo; Zamboni, Nicola; Kiefer, Patrick; et al.
  Mbio  10.1128/mBio.00390-21 Published: MAR-APR 2021
• Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle
  Keller, Philipp; Noor, Elad; Meyer, Fabian; Reiter, Michael A.; Anastassov, Stanislav; Kiefer, Patrick; Vorholt, Julia A
  Nature Communications   DOI: 10.1038/s41467-020-19235-5  OCT 26 2020
• Reciprocal growth control by competitive binding of nucleotide second messengers to a metabolic switch in Caulobacter crescentus
  Shyp, Viktoriya; Dubey, Badri Nath; Bohm, Raphael; Hartl, Johannes; Nesper, Jutta; Vorholt, Julia A; Hiller, Sebastian; Schirmer, Tilman; Jenal, Urs
  Nature Microbiology  DOI: 10.1038/s41564-020-00809-4  NOV 2020
• Microbiome manipulation by a soil-borne fungal plant pathogen using effector proteins
  Snelders, Nick C.; Rovenich, Hanna; Petti, Gabriella C.; Rocafort, Mercedes; van den Berg, Grardy C. M.; et al.
  Nature Plants  DOI: 10.1038/s41477-020-00799-5  Published: NOV 2020
• Import of Aspartate and Malate by DcuABC Drives H-2/Fumarate Respiration to Promote Initial Salmonella Gut-Lumen Colonization in Mice
  Nguyen, Bidong D.; Cuenca, Miguelangel, V; Hartl, Johannes; Guel, Ersin; Bauer, Rebekka; et al.
  Cell Host & Microbe  DOI: 10.1016/j.chom.2020.04.013  Published: JUN 10 2020
• ScalaFlux: A scalable approach to quantify fluxes in metabolic subnetworks
  Millard, Pierre; Schmitt, Uwe; Kiefer, Patrick; Vorholt, Julia A.; Heux, Stephanie; et al.
  Plos Computational Biology  https://doi.org/10.1371/journal.pcbi.1007799 APR 2020
• Molecular Basis of Growth Inhibition by Acetate of an Adenylate Cyclase-Deficient Mutant of Corynebacterium glutamicum
  Wolf, Natalie; Bussmann, Michael; Koch-Koerfges, Abigail; Katcharava, Nino; Schulte, Julia; et al.
  Frontiers In Microbiology 11 Article Number: 87 DOI: 10.3389/fmicb.2020.00087    FEB 11 2020
• Consistent host and organ occupancy of phyllosphere bacteria in a community of wild herbaceous plant species
  Massoni, Julien; Bortfeld-Miller, Miriam; Jardillier, Ludwig; Salazar, Guillem; Sunagawa, Shinichi; Vorholt JA
  Isme Journal,  DOI: 10.1038/s41396-019-0531-8   OCT 2019
• …

• Metaproteome/-genome analysis of microbial communities
• Synthetic bacterial communities
• Plant probiotics
• Identification of traits important for phyllosphere colonization
• General stress response in Alphaproteobacteria
• One carbon metabolism

• Microbial community analyses
• Proteomics
• Metabolomics
• Microscopy (FISH)
• Single cell manipulation using FluidFM technology