Reproduction is a key step in any organism's life cycle and its success relies on complex gene regulation. The fusion of highly specialized cells, the gametes, generate a totipotent embryo. We are studying the molecular mechanisms that regulate epigenetic marks, especially DNA methylation, transmission during sexual reproduction in the model plant Arabidopsis thaliana. As epigenetic marks can be modified by environmental factors, it is important to understand how DNA methylation is stably maintained while still allowing diversity in plants and other sexual organisms.

• Movement and differential consumption of short interfering RNA duplexes underlie mobile RNA interference
  Devers, Emanuel A.; Brosnan, Christopher A.; Sarazin, Alexis; Albertini, Daniele; Amsler, Andrea C; Brioudes, Florian; Jullien, Pauline E.; Lim, Peiqi; Schott, Gregory; Voinnet, Olivier
  Nature Plants  DOI: 10.1038/s41477-020-0687-2  JUL 2020
• Functional characterization of Arabidopsis ARGONAUTE 3 in reproductive tissues
  Jullien, Pauline E.; Grob, Stefan; Marchais, Antonin; Pumplin, Nathan; Chevalier, Clement; et al.
  Plant Journal  DOI: 10.1111/tpj.14868  AUG 2020
• The Diversity of Plant Small RNAs Silencing Mechanisms
  Schroder, JA; Jullien, PE
  CHIMIA, 73 (5):362-367; 10.2533/chimia.2019.362 MAY 2019
• A complex of Arabidopsis DRB proteins can impair dsRNA processing 
  Tschopp, Marie-Aude; Iki, Taichiro; Brosnan, Christopher A.; Jullien, PE; Pumplin, N
  RNA 23(5):782-797, 10.1261/rna.059519.116  MAY 2017
• DNA Methylation Influences the Expression of DICER-LIKE4 Isoforms, Which Encode Proteins of Alternative Localization and Function 
  Pumplin, Nathan; Sarazin, Alexis; Jullien, Pauline E.; et al. 
  PLANT CELL, 28 (11): 2786-2804, https://doi.org/10.1105/tpc.16.00554  NOV 2016
• DNA Methylation Dynamics during Sexual Reproduction in Arabidopsis thaliana 
  Jullien, Pauline E.; Susaki, Daichi; Yelagandula, Ramesh; et al. 
  CURRENT BIOLOGY 22(19):1825-1830, 10.1016/j.cub.2012.07.061 OCT 9 2012
• Reprogramming of DNA Methylation in Pollen Guides Epigenetic Inheritance via Small RNA 
  Calarco, Joseph P.; Borges, Filipe; Donoghue, Mark T. A.; et al. 
  CELL 151(1):194-205, 10.1016/j.cell.2012.09.001  SEP 28 2012
• DNA methylation reprogramming during plant sexual reproduction? 
  Jullien, Pauline E.; Berger, Frederic 
  TRENDS IN GENETICS 26(9):394-399, 10.1016/j.tig.2010.06.001  SEP 2010

• DNA methylation
• Sexual reproduction
• Seed development
• Small RNA