Plants use sunlight to turn carbon dioxide (CO2) and water into the sugars we eat and the oxygen we breathe. To take up CO2 from the atmosphere land plants form microscopic “breathing” pores on their leaves, which can open and close and are called stomata (Greek for “mouths”). Land plants have evolved different stomatal morphologies to improve gas exchange efficiency. Grasses like the cereals rice, maize, and wheat, for example, recruit two lateral subsidiary cells (or “helper cells”) that flank the central, dumbbell-shaped guard cells. This morphology allows grass stomata to open and close faster and, thus, save water.
Our lab wants to understand (1) how the innovative grass stomata develop, (2) how their innovative form supports fast opening and closing, and (3) if we can engineer stomatal form to improve water use efficiency and stress resilience. To this end, we use CRISPR/Cas9 gene-editing, (time-lapse) confocal microscopy, (single-cell) transcriptomics, and leaf-level gas exchange measurements. We primarily work with the genetic model grass Brachypodium distachyon – a wild relative of wheat and barley –which we can transform efficiently using embryonic tissue culturing.